Experience Summary
Molecular Biology
* Gene-Chip technology, Sample preparation for Gene-Chip Gene-Chip Data-Mining.
* Real time PCR, RT-PCR, Competitive PCR, relative and quantitative real time PCR.
* Screening expression library, DNA sequencing, cloning, mobility gel shift assays, and making Triplex and Duplex affinity columns.
* Mammalian cell culture, Cell culture techniques involving cell fusion, transfection, and selection by drugs.
* RNA/DNA, miRNA isolation, in situ hybridization.
Protein
* Protein purification, HPLC and FPLC.
* Production of monoclonal antibodies, antisera purification, Western blots, and immunoprecipitation.
Staff Training
*OSHA safety regulations, IRB protocol, Standard common lab techniques
Project Management
*Gene-Chip Data, analysis, and Real time PCR management, Guiding and directing the team of six graduate and medical students with the result of five published papers in peer reviewed journal.
Animal Handling
*Handling mice for different studies, control vs treated.
Resolving trouble shooting skills
*Resolving technique trouble shooting protocols.
Team Leadership
*In-depth characterization and linkage of the DNA/ RNA/miRNA probes on the Affymetrix Gene-Chips and their relevance with the analyzed data
*Gene-Chip data analysis, know and how to start and finish with a published paper.
Teaching Style
First -Meet the student, Assess the strength and weakness of the student.
Second Using the students strength strategy work on the weakness.
I believe in doing, for example assignments, lectures have to be interactively approached and conducted independently by the student at all times.
My Hobbies
Cooking, Gardening, sewing, Reading books, Learning new Computer skills.
Other Comments
International Travel only in First Class.